Establishment and characterisation of cell lines from the caerulean damsel, Pomacentrus caeruleus

Cell lines and primary cell cultures from fish tissues have been used for research in disease diagnosis and cytotoxicity evaluation of environmental pollutants. The principal aim of this work was the development of continuous cell lines from the caerulean damsel, Pomacentus caeruleus. To achieve this, tissues were first sourced from donor fishes, devoid of contaminants using standardised disinfection protocols. Primary cultures were then initiated and suitable media, additives, dissociation reagents as well as optimum incubation temperature were determined. Successful primary cultures were subcultured and passaged to derive continuous cell lines which were cryopreserved for long term storage. The continuous cell lines established were characterised by immunotyping using cell type markers and authenticated to confirm the species of origin using previously established barcoding techniques. Preliminary applications such as gene transfection studies and cytotoxicity assays using bacterial extracellular products were done in addition to ensuring that the cultures and cryostocks were contamination-free

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Not AvailableExplantation and trypsinisation methods for tissue dissociation were attempted for the establishment of primary cell cultures from the caerulean damsel, Pomacentrus caeruleus. Among the tissues taken, fin, liver and caudal peduncle showed good attachment with emergence of cells. The cells were best suited to grow in Leibovitz’s L-15 basal medium supplemented with foetal bovine serum (initially 20 % which was later reduced to 5–10 % during subsequent passages) at an ambient temperature of 28 ± 2 �C and pH 7.2 ± 0.2. These cultures persisted at temperatures from 17 to 32 �C, and proliferated at temperatures from 24 to 30 �C. The cells have been cryopreserved successfully with a survival rate of 80 %. Results suggest that fin, caudal peduncle and liver cell cultures have potential for development into cell lines.Not Availabl

Evaluation of Various Tissues of the Caerulean Damsel, Pomacentrus caeruleus for Initiating In Vitro Cell Culture Systems

Explantation and trypsinisation methods for tissue dissociation were attempted for the establishment of primary cell cultures from the caerulean damsel, Pomacentrus caeruleus. Among the tissues taken, fin, liver and caudal peduncle showed good attachment with emergence of cells. The cells were best suited to grow in Leibovitz’s L-15 basal medium supplemented with foetal bovine serum (initially 20 % which was later reduced to 5–10 % during subsequent passages) at an ambient temperature of 28 ± 2 �C and pH 7.2 ± 0.2. These cultures persisted at temperatures from 17 to 32 �C, and proliferated at temperatures from 24 to 30 �C. The cells have been cryopreserved successfully with a survival rate of 80 %. Results suggest that fin, caudal peduncle and liver cell cultures have potential for development into cell lines

Derivation, Characterization and Cryostorage of continuous cell lines from threatened species of groupers(Serranidae) Cromileptes altivelis and Epinephelus bleekeri

Derivation, Characterization and Cryostorage of continuous cell lines from threatened species of groupers(Serranidae) Cromileptes altivelis and Epinephelus bleeker

Successful cryostorage of twenty authenticated cell lines derived from eight species of marine fish maintained over a period of more than five years

Successful cryostorage of twenty authenticated cell lines derived from eight species of marine fish maintained over a period of more than five year

Development of Cell culture systems from the Caerulean Damsel, Pomacentrus cauruleus (Quoy,& Gaimard, 1825)

Development of Cell culture systems from the Caerulean Damsel, Pomacentrus cauruleus (Quoy,& Gaimard, 1825

In vitro culture and characterisation of Embryonic Stem (ES)cells from the maroon clown fish Premnas biaculeatus (Bloch, 1790)

In vitro culture and characterisation of Embryonic Stem (ES)cells from the maroon clown fish Premnas biaculeatus (Bloch, 1790